Product Data Sheet - ProDx trap columns
Part number: PD-1149-G
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LEAP HDX automation
Contact a workflow specialist for your specific needs
Proven, complete solution
HDX has become an indispensable tool for protein binding studies in pharmaceutical development. For over 15 years Trajan's automation team (formally LEAP Technologies) have been automating HDX experiments, and is the industry leader in the field.
LEAP HDX systems are helping researchers across the globe come to answers about their proteins faster than traditional methods.
Features and benefits
Successful HDX experimentsHydrogen Deuterium Exchange is an experimental technique for obtaining information about changes in the tertiary structure of proteins under different physiological conditions. Automation addresses the 4 key factors for successful HDX experiments:
- Accurate timing
- pH control
- Sustained 0°C environment
- In-line digestion
Faster answers
Powerful information on protein structure in days - not weeks. Move more quickly, make better decisions.
Recommended applications
Automated Hydrogen-Deuterium Exchange (HDX) experiments.
Our turn-key HDX solution is compatible with most MS analyzers, and can extend your current LC/MS capabilities while adding a new dimension to your protein studies.
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LEAP HDX automation
The LEAP HDX platform is an advanced, automated scheduling and experimental workstation providing ease of use, high reproducibility and exceptional data quality for HDX experiments.Labeling
HDX relies on accurate timing of the labeling step.Quenching
The system will optimize the timing of the labeling step to ensure quenching is just in time for the LC-MS injection, using a chilled syringe. A typical experiment might consist of a dozen time points, in triplicate, for each protein sample. The LEAP HDX system will schedule the experiments and perform all steps for each time point of every sample. Long labeling experiments are automatically scheduled first to ensure the shortest possible instrument time.Optimized HDX fluidics
The introduction of optimized HDX fluidics that enables full fluidic path backflushing, extending column life and maintaining consistent retention times to deliver higher quality data.The new fluidics pathway is built upon UHPLC rated valves and column hardware (21 kpsi), with flow paths minimized to reduce swept volumes.
Trajan manufactured HDX specific pre-cut, high pressure lines and fittings, designed to improve reliability and enhance performance. All fluidic components are designed to offer consistently reproducible results.
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ChronosHDX control software
Meeting the challenge of producing the highest quality data sets.HDX experiments require time-sensitive operation. Therefore, in traditional methods, an operator has to attend throughout the course of HDX experiments. However, time-controlled automation enables unattended operation using intelligent scheduling with Chronos software.
Trajan uses ChronosHDX scheduling software to automate the precise labeling and quenching of samples, as well as injection and digestion of proteins on the LC-MS analysis system.
- Precise on-exchange data point timing
- Reproducible experimental conditions
- Multi-valve control for trapping and elution of digested peptides to the LCMS system
- Throughput optimization with elimination of timing conflicts
- Simple graphic user interface
- Advanced method editing capabilities
- Integrated with HDExaminer data processing software from Sierra Analytics
HDExaminer
Native data format compatibility with HDExaminer increases high confidence data and allows you to get to results faster.- HDExaminer finds accurate results even with overlapping peptides.
- The software works well with low resolution or high resolution data.
- Imports MS data from Agilent, AB Sciex, Bruker, Thermo, or Waters instruments, as well as mzXML or mxData.
- Imports peptide search data from SEQUEST HTML, Mascot XML, Proteome Discover xls or pepXML.
Efficient system control and optimized experimental design
Our Chronos for HDX software now offers a streamlined interface for HDX users and automatically overlaps time points for maximum MS efficiency.Temperature control at all steps ensures reliable data. Sustained 0°C environment reduces back exchange, improves sample stability and overall reproducibility.
Our most flexible automation platform yet offers the ability to run multiple experimental methods in the same sequence. Native data format compatibility with HDExaminer increases high confidence data and allows you to get to results faster.
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LEAP HDX specifications On-exchange time range 10 seconds to 4+ hours, user selectable Temperature control ±1.0°C Temperature range 0°C to 37°C Volumetric range Typically 1-250 μL, use application selectable to 5000 μL Electrical 120 VAC, 60 Hz or 220 VAC, 50 Hz Dimensions 80 cm (W) x 38.5 cm (D) x 64.8 cm (H) Weight 80 kg LCMS data systems Compatible with nearly all LCMS data systems. Direct integration with the most common. -
Service you can trust
Our CTC Manufacturer Certified Service team is ready to keep your automation at its peak performance.Full application support
Trajan is the leading expert in automation of HDX. Our in-house specialists and team of experienced partners offer you the best consulting available to achieve your HDX goals.Expert training
Trajan has the expertise and experience with automating HDX experiments that can only come from having worked with leading scientists in the field. -
ProDx consumables designed for HDX
Columns and fluidics consumables specifically designed to optimize all aspects of the HDX user experience.View the range of ProDx columns
ProDx Protease columns
ProDx consumables are specifically designed and developed for use with the LEAP HDX platform.
The ProDx Protease column is optimized to perform at 0°C, the ideal temperature for performing HDX reactions.
Improved protease coupling reduces carryover from non-specific binding, while lower backpressures minimize the potential for contamination and overpressure leakage.
When used in combination with the new LEAP HDX valve system, it can be backflushed automatically to remove frit-loaded or undigested intact material. Clean columns give consistent retention times, overcoming traditional issues with non-selective binding and poor reproducibility.
To ensure confidence in your data, combine the ProDx Protease and trap columns for your HDX work
Features and benefits
- Improve workflow efficiencies and reduce instrument downtime
- Low temperature operation optimized at 0°C
- Lower carryover and increased desalting efficiency
- High pressure hardware designed for continuous use at 20,000 psi (1,379 bar)
- More overlapping fragments for high confidence data
- Automated backflushing when used with the LEAP HDX system
Recommended applications
Automated Hydrogen-Deuterium Exchange (HDX) experiments using the LEAP HDX platform.
Product specifications
ProDx columns were developed specifically for use with the LEAP HDX system. Packed in bio-inert stainless steel glass lined columns to withstand high pressure digestion practices, making it compatible with other systems.
- ID configuration: 2.1 mm
- Length configuration: 30 mm
The ProDx Protease (Pepsin) column is the first in a range of immobilized Protease columns available from Trajan. The initial intended use is with the LEAP HDX workflow, where these columns are used in-line for protein-protein and protein-ligand studies. The columns can be deployed outside of the HDX workflow as appropriate, possible alternate configurations might include general proteomics workflows, protein purification studies and QC applications. Column chemistry is such that limited exposure to common denaturing reagents and quenching salts (such as 6.4M Guanidine Hydrochloride, 8M Urea and 4M TCEP) do not adversely impact proteolytic performance.
ProDx Protease (Pepsin) columns are delivered chilled for optimal performance. Columns need to be maintained between 2-8°C. Column performance can be reduced if the column is maintained and operated above 15°C for extended periods of time, or if exposed to non-acidified aqueous reagents or organic reagents.
| Phase | Pore size (Å) | Particle size (µm) |
| Protease (Pepsin)* | 500-10,000 Å | 20 μm |
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Please refer to the part number list for more items.
*This product contains POROS® material used under license for Sample Prep purposes from Life Technologies Corporation, a subsidiary of Thermo Fisher Scientific, Inc. The POROS® Trademark is owned by Thermo Fisher Scientific, Inc.
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ProDx trap columns
ProDx consumables are specifically designed and developed for use with the LEAP HDX platform.
ProDx trap columns are optimized for maximum recovery of peptide fragments, efficient desalting and low backpressures during the digestion and elution processes of HDX runs.
While many columns used for HDX trapping are not designed for use with low temperature HDX systems, ProDx trap columns are designed to operate down to 0°C, with consistent recovery, and demonstrate reduced backpressure at standard conditions of 200 μL/min, 100% Aqueous and 0°C, lowering instances of leakage and need for troubleshooting.
ProDx trap columns are pre-configured with PEEKsil® lines for ease of use, reducing the number of fittings needed in the system, preventing issues that commonly occur when relying on other columns with multiple connections.
To ensure confidence in your data, combine the ProDx Protease and trap columns for your HDX work
Features and benefits
- High recovery of peptide fragments generated by inline digestion
- Does not interfere with analytical separation
- Low temperature operation optimized at 0°C
- Lower carryover and increased desalting efficiency
- PEEKsil lines for reduced backpressure and leakage
- End frits designed to prevent rapid blocking by intact proteins and aggregates
- Phase and pore size to suit your analysis
Recommended applications
Automated Hydrogen-Deuterium Exchange (HDX) experiments using the LEAP HDX platform.
Product specifications
ProDx columns were developed specifically for use with the LEAP HDX system. ProDx trap columns are designed to protect the ProDx analytical column.
- ID configuration: 0.5 mm
- Length configuration: 20 mm
Currently available in C8 Analog*, with performance similar to C18 materials, suitable for reversed-phase separation of biomolecules such as proteins and peptides.
Fittings for ProDx trap columns
Choice of fittings available with ProDx trap columns, for low to ultra high pressure applications.
ProteCol® PEEK Fingertight fittings are precision manufactured to complement your HPLC needs.
| ProteCol® PEEK Fingertight HPLC fittings | Pressure rating | Maximum operating pressure rating when used with ProDx trap columns |
| 1/16" to 10-32 UNF (long) | 5,000 psi (345 bar) | 5,000 psi (345 bar) |
| 1/32" to 6-40 UNF | 2,750 psi (190 bar) | 2,750 psi (190 bar) |
PEEKLok™ UHPLC fittings are designed specifically for PEEKsil tubing: reliable, affordable, and easy to use, holding up to 22,000 psi; or up to 15,000 psi when used with ProDx trap columns.
| PEEKLok™ UHPLC fittings | Pressure rating | Maximum operating pressure rating when used with ProDx trap columns |
| 1/16" to 10-32 UNF | 22,000 psi (>1,500 bar) | 15,000 psi (1,000 bar) |
| 1/32" to 6-40 UNF | 22,000 psi (>1,500 bar) | 15,000 psi (1,000 bar) |
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Please refer to the part number list for more items.
*This product contains POROS® material used under license for Sample Prep purposes from Life Technologies Corporation, a subsidiary of Thermo Fisher Scientific, Inc. The POROS® Trademark is owned by Thermo Fisher Scientific, Inc.
View full product details
LEAP infusion automation
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Expert infusion automation for your high sensitivity metabolomics and lipidomics experiments
High-throughput, high sensitivity and low carryover: the new infusion automation from Trajan offers you everything you need to bring your lipidomics mass spec workflow to its peak performance.
Move from manual sample infusion to the highest standard of infusion automation. With rapid cleanup between injections and best-in-class software architecture to integrate into your workflow, our infusion automation will elevate your lipidomics experiments.
Features and benefits
- High throughput
- High sensitivity
- Low carryover
The most consistent and flexible system available for infusion automation
Fully customizable infusion eliminates carryover concerns while saving you time. Allows for sample infusion at flow rates between 5 and 100 µL/min — directly into your ESI source via the injection valve — precisely and without pulsation. Compatible with both shotgun and targeted lipid analysis.
The powerful Chronos software can execute important operational and integration functions such as reading and reporting to LIMS software, as well as MS acquisition mode switching within a single infusion. The system maintains flow to the source while quickly and thoroughly washing the syringe system for the next infusion.
Exclusive inert configuration is available for your trickiest lipid classes — ensuring your samples never come into contact with any polymers.
“We just ran a whole pile of samples. It ran 24/7 without a hitch. Very reliable.” - Calvin Vary, PhD, Proteomics and Lipidomics Core Facility Maine Medical Center Research Institute, Maine, USA.
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Upgrade sample throughput without worrying about carryover
The LEAP PAL injector head is a syringe pump itself, making it the ideal sample delivery system for ESI flow rates, unlike conventional HPLC pumps.Many lipids are notorious for their adherence to glass and other surfaces resulting in carryover between samples. The special LCMS syringe tool incorporates unique features to minimize this carryover to below 1% for the abundant neutral and phospholipids and even lower for other species.
The illustration below shows how this rapid washing is achieved. After washing the inside, the system will wash the needle externally and be ready to aspirate the next sample.
Figure 1 Sample infusion -
Greatest experiment control with powerful, user-friendly software
The infusion process is controlled using our Chronos software, allowing maximum compatibility with your preferred Chromatography Data System, LIMS and MS data systems.Method parameters are available for flow rates, infusion times, stabilization delays and cleanup conditions, MS acquisition method switching.
With an optional second valve, standard chromatography methods can be used in the same sequence with direct infusion samples.
Figure 2 An example Chronos infusion method -
Specifications Flow rate range 5-100 μL/min. Sample formats Sealed and unsealed vials (1 mL, 2 mL, 10 mL); sealed and unsealed plates (96 and 384-well). Optional features Sample cooling drawers (4-40°C), inert flow path, second valve for chromatography autosampling Sample capacity Expandable capacity, from three plate tray holder, to 6 or 12 plate drawer stack(s). Capacity depends on vial/tray formats and drawer configuration, maximum 4 stacks Signal stability Signal stability on a single sample has been demonstrated for 20 minutes. Long term stability confirmed in successful analysis of 400 samples over a 36 hour period (5 minute injection per sample). Computer requirements (minimum) Operating system: Windows 7 SP1 (32 or 64 bit), Windows 8.1 or Windows 10. Hardware: Intel Dual Core 2.0 GHz, 60 GB hard disk, 4 GB RAM, dedicated ethernet port or free location on local private network switch with 1 free USB port for security dongle. Monitor: 1366 x 768 (recommended 1440 x 900 or 1920 x 1080).