Part number: PDX.T8H02-32
Image is representative (may not be specific item).
ProDx consumables are specifically designed and developed for use with the LEAP HDX platform.
ProDx trap columns are optimized for maximum recovery of peptide fragments, efficient desalting and low backpressures during the digestion and elution processes of HDX runs.
While many columns used for HDX trapping are not designed for use with low temperature HDX systems, ProDx trap columns are designed to operate down to 0°C, with consistent recovery, and demonstrate reduced backpressure at standard conditions of 200 μL/min, 100% Aqueous and 0°C, lowering instances of leakage and need for troubleshooting.
ProDx trap columns are pre-configured with PEEKsil® lines for ease of use, reducing the number of fittings needed in the system, preventing issues that commonly occur when relying on other columns with multiple connections.
To ensure confidence in your data, combine the ProDx Protease and trap columns for your HDX work
Automated Hydrogen-Deuterium Exchange (HDX) experiments using the LEAP HDX platform.
ProDx columns were developed specifically for use with the LEAP HDX system. ProDx trap columns are designed to protect the ProDx analytical column.
Currently available in C8 Analog*, with performance similar to C18 materials, suitable for reversed-phase separation of biomolecules such as proteins and peptides.
Fittings for ProDx trap columns
PEEKLok™ UHPLC fittings are designed specifically for PEEKsil tubing: reliable, affordable, and easy to use, holding up to 22,000 psi; or up to 15,000 psi when used with ProDx trap columns.
| PEEKLok UHPLC fittings | Pressure rating | Maximum operating pressure rating when used with ProDx trap columns |
| 1/16" to 10-32 UNF | 22,000 psi (>1,500 bar) | 15,000 psi (1,000 bar) |
Please refer to the part number list for more items.
*This product contains POROS® material used under license for Sample Prep purposes from Life Technologies Corporation, a subsidiary of Thermo Fisher Scientific, Inc. The POROS® Trademark is owned by Thermo Fisher Scientific, Inc.
Contact a workflow specialist for your specific needs
Since 2002 Trajan has been automating HDX, providing continuous enhancements for rugged HDX sample control and scheduling.
Trajan’s LEAP HDX system is the recognized leader, preferred around the globe by scientists requiring confidence in reproducible results.
Automated HDX provides structural confidence in native states
HDX, especially automated HDX, has uses in development, manufacturing and post-production, providing insight into your protein’s tertiary structure, in its native state(s).
Successful HDX experiments are all about control
With robotic precision, Trajan’s HDX platform exerts exacting control of all key factors:
HDX is a well-established analytical technique for protein-ligand, protein-protein, protein-conjugate interactions and protein stability studies.
HDX uses variations in deuterium exchange rates between more and less accessible/structured regions throughout a protein.
In addition to being faster than other protein techniques like x-ray crystallography and NMR, HDX data can be obtained from samples that are challenging to crystalize and from samples in short supply. It can be used to replicate critical native state conditions, for the most useful results. HDX is an indispensable complementary tool for protein biophysics.
Trajan’s HDX system is compatible with bottom-up, top- and middle-down protein analysis studies.
The total HDX platform
Our total HDX platform covers the complete process: sample input >> the many meticulous steps of preparation >> optimizing mass spec utilization >> clear answers with minimal manual data curation.
Faster answers
Powerful information on protein structure in days – not weeks. Move more quickly, make better decisions.
With HDExaminer, swiftly and easily analyze data from any MS instrument to get a complete picture of the overall deuteration behavior of your protein.
Automation at its finest
The most efficient system control, with unique temperature set points at all stages of the experiment to ensure reliable data. Sustained 0°C environment reduces back exchange, improves sample stability and overall reproducibility.
Our Chronos for HDX software offers a streamlined interface for HDX users and automatically overlaps time points for maximum MS efficiency.
Combines ease of use with advanced options, allowing promotion of user-defined parameters to the sample list, giving the operator control over all aspects of the method without complication.
HDExaminer: a complete analytical tool for HDX experiments
The Trajan LEAP HDX platform works natively with HDExaminer from Sierra Analytics, a complete software solution for HDX analysis, independent of MS data type.
HDExaminer is designed with ease of use in mind, offering numerous ways to visualize your data. These include:
Unique fluidics automation for the best performance
Trajan’s LEAP HDX platform incorporates an advanced cooling chamber for the trapping and chromatography phases which includes an enclosed valve and column chiller as well as solvent pre-conditioning.
Digestion temperature is controlled independently with the protease column chamber, to allow improved digestion efficiency.
A special 3 valve configuration enables zero-downtime backflushing for retention time stability, extended column life and superior carryover protection.
Optimized fittings and precut tubing ensure maximum reliability and instrument up-time.
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Specifications |
Compact |
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Parallel (standard) |
Parallel (extended) |
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Automatic syringe selection for extended dynamic range |
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Chilled syringe for quench/injection |
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Vial capacity for 100 time point experiments |
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Optimized valve fluidics for inline digestion and automatic backflushing |
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Exchange time points down to 30 seconds |
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Independent protease column temperature control |
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Trajan HDX syringes |
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Trajan ProDx protease columns optimized for low temperature |
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Trajan ProDx trap columns optimized for low temperature |
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Dual head for simultaneous operations |
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Exchange time points down to 15 seconds |
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Forward compatible capacity for future models |
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Optional |
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Secondary chilled syringe for protein handling |
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Optional |
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Membrane protein filtration |
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Optional |
Optional |
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96 or 384 well microtiter plate capacity |
Optional
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Optional |
Optional |
Optional |
Expert training
Trajan Scientific and Medical is the global expert in HDX automation. Our in-house specialists and experienced partners offer the best training and consulting available to achieve your HDX goals.
Supporting more operation modes in more places than ever
With 40 years combined experience in HDX and coupled to our unparalleled relationships with the global leaders in the technique worldwide, our HDX automation continues to offer application support for an ever-expanding portfolio of options.
New features and integrations now offer you better access to membrane proteins, proteins at biologically relevant temperatures, and even more flexible protein regions than ever before.
The Trajan LEAP HDX platform is supported directly by Trajan in Europe, Australia, Japan and the USA, and through partners in the Middle East, India and beyond.
Service you can trust
Our CTC manufacturer-certified service team is available to assist you in person, by phone and remote session.
Now offering local time zone support in US, EU, ANZ and Asiatic time zones.
Available extended support contracts, training and preventative maintenance schedules.
Consumables designed for HDX
Columns and fluidics specifically designed to optimize all aspects of the HDX user experience. From ease of use to reliability and sample stability, your success is our number one goal.
ProDx Protease columns are designed to perform with lower carryover, lower backpressure, and better digestion characteristics at 0°C for longer lifetimes.
ProDx Trap columns are uniquely engineered to operate under HDX experimental conditions.
View the range of ProDx columns
ProDx consumables are specifically designed and developed for use with the LEAP HDX platform.
The ProDx Protease column is optimized to perform at 0°C, the ideal temperature for performing HDX reactions.
Improved protease coupling reduces carryover from non-specific binding, while lower backpressures minimize the potential for contamination and overpressure leakage.
When used in combination with the new LEAP HDX valve system, it can be backflushed automatically to remove frit-loaded or undigested intact material. Clean columns give consistent retention times, overcoming traditional issues with non-selective binding and poor reproducibility.
To ensure confidence in your data, combine the ProDx Protease and trap columns for your HDX work
Automated Hydrogen-Deuterium Exchange (HDX) experiments using the LEAP HDX platform.
ProDx columns were developed specifically for use with the LEAP HDX system. Packed in bio-inert stainless steel glass lined columns to withstand high pressure digestion practices, making it compatible with other systems.
The ProDx Protease (Pepsin) column is the first in a range of immobilized Protease columns available from Trajan. The initial intended use is with the LEAP HDX workflow, where these columns are used in-line for protein-protein and protein-ligand studies. The columns can be deployed outside of the HDX workflow as appropriate, possible alternate configurations might include general proteomics workflows, protein purification studies and QC applications. Column chemistry is such that limited exposure to common denaturing reagents and quenching salts (such as 6.4M Guanidine Hydrochloride, 8M Urea and 4M TCEP) do not adversely impact proteolytic performance.
ProDx Protease (Pepsin) columns are delivered chilled for optimal performance. Columns need to be maintained between 2-8°C. Column performance can be reduced if the column is maintained and operated above 15°C for extended periods of time, or if exposed to non-acidified aqueous reagents or organic reagents.
| Phase | Pore size (Å) | Particle size (µm) |
| Protease (Pepsin)* | 500-10,000 Å | 20 μm |
Please refer to the part number list for more items.
*This product contains POROS® material used under license for Sample Prep purposes from Life Technologies Corporation, a subsidiary of Thermo Fisher Scientific, Inc. The POROS® Trademark is owned by Thermo Fisher Scientific, Inc.
View full product details
Contact a workflow specialist for your specific needs
High-throughput, high sensitivity and low carryover: the new infusion automation from Trajan offers you everything you need to bring your lipidomics mass spec workflow to its peak performance.
Move from manual sample infusion to the highest standard of infusion automation. With rapid cleanup between injections and best-in-class software architecture to integrate into your workflow, our infusion automation will elevate your lipidomics experiments.
The most consistent and flexible system available for infusion automation
Fully customizable infusion eliminates carryover concerns while saving you time. Allows for sample infusion at flow rates between 5 and 100 µL/min — directly into your ESI source via the injection valve — precisely and without pulsation. Compatible with both shotgun and targeted lipid analysis.
The powerful Chronos software can execute important operational and integration functions such as reading and reporting to LIMS software, as well as MS acquisition mode switching within a single infusion. The system maintains flow to the source while quickly and thoroughly washing the syringe system for the next infusion.
Exclusive inert configuration is available for your trickiest lipid classes — ensuring your samples never come into contact with any polymers.
“We just ran a whole pile of samples. It ran 24/7 without a hitch. Very reliable.” - Calvin Vary, PhD, Proteomics and Lipidomics Core Facility Maine Medical Center Research Institute, Maine, USA.
Upgrade sample throughput without worrying about carryover
The LEAP PAL injector head is a syringe pump itself, making it the ideal sample delivery system for ESI flow rates, unlike conventional HPLC pumps.
Many lipids are notorious for their adherence to glass and other surfaces resulting in carryover between samples. The special LCMS syringe tool incorporates unique features to minimize this carryover to below 1% for the abundant neutral and phospholipids and even lower for other species.
The illustration below shows how this rapid washing is achieved. After washing the inside, the system will wash the needle externally and be ready to aspirate the next sample.
Figure 1 Sample infusion
Greatest experiment control with powerful, user-friendly software
The infusion process is controlled using our Chronos software, allowing maximum compatibility with your preferred Chromatography Data System, LIMS and MS data systems.
Method parameters are available for flow rates, infusion times, stabilization delays and cleanup conditions, MS acquisition method switching.
With an optional second valve, standard chromatography methods can be used in the same sequence with direct infusion samples.
Figure 2 An example Chronos infusion method
| Specifications | |
|---|---|
| Flow rate range | 5-100 μL/min. |
| Sample formats | Sealed and unsealed vials (1 mL, 2 mL, 10 mL); sealed and unsealed plates (96 and 384-well). |
| Optional features | Sample cooling drawers (4-40°C), inert flow path, second valve for chromatography autosampling |
| Sample capacity | Expandable capacity, from three plate tray holder, to 6 or 12 plate drawer stack(s). Capacity depends on vial/tray formats and drawer configuration, maximum 4 stacks |
| Signal stability | Signal stability on a single sample has been demonstrated for 20 minutes. Long term stability confirmed in successful analysis of 400 samples over a 36 hour period (5 minute injection per sample). |
| Computer requirements (minimum) | Operating system: Windows 7 SP1 (32 or 64 bit), Windows 8.1 or Windows 10. Hardware: Intel Dual Core 2.0 GHz, 60 GB hard disk, 4 GB RAM, dedicated ethernet port or free location on local private network switch with 1 free USB port for security dongle. Monitor: 1366 x 768 (recommended 1440 x 900 or 1920 x 1080). |
Contact a workflow specialist for your specific needs
Zero-loss, zero-drip, high throughput and ultra reliable: the new microfraction collection system from the Automation team at Trajan (formally LEAP Technologies) offers you everything you need to consistently perform your most critical collection experiments.
The LEAP MFx collector has been purposefully designed to optimize fraction collection for the sharpest peaks, the maximum dependability, and the greatest ease of use. With smart software features and automation, this analytical fraction collection system is the most reproducible, and highly compatible platform available today.
The highest capacity, most flexible system available
Fully customizable collection into a variety of plate and vial types, including sealed container collection: 96 and 384 well plate formats, 2 mL and 10 mL vial formats (sealed or open), and 2 mL and 10 mL tube formats.
Compatibility with a broad range of analytical flow rates, sub-ambient temperature control, and user-friendly software are just a few of the features that make this new system the perfect evolution for your fraction collecting needs.
Automated microfraction collection experiments.
Advanced hardware
The most flexible system on the market, the LEAP MFx collector solution is available:
| Plate capacity | Up to 12 plates | Up to 24 plates |
| Instrument width | 50 cm | 120 cm |
Designed for safety and serviceability
| LEAP MFx collector | Competitor 1 | Competitor 2 | |
|---|---|---|---|
| Zero-loss collection (doesn’t divert to waste) | Yes | No | No |
| Cooling to 4°C | Yes | Yes | No |
| Maximum capacity | |||
| Deep well plates | 24 | 4 | 8 |
| Shallow well plates | 48 | 4 | 8 |
| Vials/tubes | 24 trays (up to 1296 tubes/vials) | 215 tubes | 768 tubes |
Unique dynamic flow reservoir
The precision engineered and long lasting dynamic flow reservoir (DFR) allows the LEAP MFx collector to continue collecting chromatographic flow while the automation is moving between wells, resulting in zero-loss.The design achieves a turbulence-free, fully optimized flowpath for collection without peak dispersion.
Undisturbed flowpath for minimal peak diffusion
While flow to the buffer region remains constant during both well filling and robot movement, the buffer region will expand as the DFR travels between wells. This retracts any hanging droplets, and avoids peak diffusion by allowing continuous flow. The buffer region contracts to resume dispensing at the next well.
Setup for collection has never been easier
Tabular and visual method creation
Maintains highest peak resolution with sub-2 second fractions, while providing simplified data correlation, and advanced sample tracking
or serpentine by rows 
Smart features offer convenience and reduce error
| LEAP MFx collector specifications | |
|---|---|
| Maximum flow rate | >5 mL/min |
| Minimum dwell time | <2 seconds/well |
| Collection formats | 96 and 384 well plates (deep and shallow), 2 mL and 10 mL vials/tubes |
| Sealed collection | Can pierce sealed plates and sealed vials |
| Computer requirements (minimum) | Operating system: Windows 7 SP1 (32 or 64 bit), Windows 8.1 or Windows 10. Hardware: Intel Dual Core 2.0 GHz, 60 GB hard disk, 4 GB RAM, dedicated ethernet port or free location on local private network switch with 1 free USB port for security dongle. Monitor: 1366x768 (recommended 1440x900 or 1920x1080). |
Service you can trust
Our CTC Manufacturer Certified Service team is ready to keep your fraction collection automation at its peak performance.
Full application support
Trajan is the leading expert in automation of fraction collection for analytical pursuits. Our in-house specialists and team of experienced partners offer you the best consulting available to achieve your microfraction collection goals.
Expert training
Trajan has the expertise and experience with automating fraction collection experiments that can only come from having worked with leading scientists in the field.
Reliable engineering and consumables for sharp peaks and reproducibility
Tubing replacement kits with pre-cut tubing, optimized fittings and ferrules ensure that the MFx collector, built on the trusted PAL platform, is the most advanced fraction collection automation for zero carryover, sharper peaks and stunning reproducibility.
View the range of LEAP MFx collector accessories
An example of actual sample collection with the LEAP MFx collector, showing baseline resolution and well-defined peaks
