Training - GC column phases

BP Capillary Columns 

Features to look for when Purchasing a Column

  • Low bleed
  • Maximum temperatures
  • Column inertness
  • Resistance to chemical degradation, air and water
  • Produces needed results

SGE Stationary Phases

  • BP - Conventional technology
  • BPX - High performance / high temperature
  • HT - Very high temperature Capillary Columns
  • Wax

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BP Capillary Columns

BP Columns have polysiloxane based phases.

>>>> phase_bp1 image

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BPX - High performance/High temp

Features to look for when Purchasing a Column

  • Low bleed
  • Maximum temperatures
  • Column inertness
  • Resistance to chemical degradation, air and water
  • Produces needed results

SGE Stationary Phases

  • BP - Conventional technology
  • BPX - High performance / high temperature
  • HT - Very high temperature Capillary Columns

Features of BPX Columns:

  • BPX has the highest temperature rating on the market
  • Dramatically increases the range of analysis
  • Longer column lifetimes
  • BETTER PERFORMANCE ms grade column for routine applications higher operating temperatures column inertness
  • LONGER COLUMN LIFE lower bleed greater column inertness resistance to oxidation by air
  • LOW COLUMN BLEED
  • EXTENDED TEMPERATURE
  • RESISTANT TO AIR OXIDATION
  • CHROMATOGRAPHIC INERTNESS

BPX Chromatograms

Basic Rules of Column Selection

  • Know your components
  • Use the least amount of theoretical plates as possible (uses less time)
  • Know your phases: maximum temps, polarities
  • Know your basic rules of chromatography
  • If you have one in your cupboard - try it!
  • Look at the literature
  • Ask SGE
  • If in doubt, use a: 30 M x 0.25 mm ID 0.25 micron BPX5

BPX Capillary Columns


BPX5 Min: -40°C
Max: 360/ 370°C
Non-Polar
5% Phenyl polysilphenylene-siloxane

BPX35 Min: 0°C
Max: 360/370°C
Slightly-Polar
35% Phenyl polysilphenylene-siloxane

BPX-VOLATILES Min: 0°C
Max: 290/300°C
Slightly-Polar

BPX50 Min: 80°C
Max: 330/350°C
Moderately-Polar
50% Phenyl polysilphenylene-siloxane

BPX70 Min: 50°C
Max: 260/290°C
Polar
70% Cyanopropyl polysilphenylene-siloxane

BPX90 Max: 280°C
Polar  
90% Cyanopropyl polysilphenylene-siloxane

Features of BPX Column >>

 

What are the features of a BPX column?

SGE was the first company to introduce polysiloxane stationary phases modified with the silphenylene unit. This innovation ushered in the new era of low bleed, high maximum operating temperature columns.

  • Silphenylene phase coated onto fused silica column wall
  • Bonded/ crosslinked to improve life and stability
  • Highest operating temperatures on the market
  • Low column bleed necessary for GC/ MS detection levels
  • Stability in the presence of water and air in a carrier gas

These features make BPX superior to all MS grade and exceptionally low bleed columns on the market.

BPX High Performance Capillary Columns


BPX1 100% Polydimethylsiloxane

Equivalent to: DB- 1, HP- 1, HP- 1MS, HP- Ultra 1, Rtx- 1, CP- SIL 5CB, CP- SIL 5CB- MS, SPB- 1, OV- 1, PE- 1, 007- 1( MS), SP- 2100, SE- 30, ZB- 1


BPX5 5% Diphenyl 95% Dimethyl Polysilphenylene-siloxane

Total phenyl content of phase is 5%

Equivalent to: DB- 5MS, DB- 5.625, HP- 5TA, XTI- 5, CP- SIL 8CBms, MDN- 5S


BPX35 35% Diphenyl 65% Dimethyl Polysilphenylene-siloxane

Total phenyl content of phase is 35%

Equivalent to: DB- 35MS, PE- 35ms, HP- 35MS, MDN- 35

Replaces: HP- 35, DB- 35, Rtx- 35, SPB- 35, PE- 35, AT- 35


BPX50 50% Diphenyl 50% Dimethyl Polysilphenylene-siloxane

Total phenyl content of phase is 50%

Equivalent to: DB- 17MS, HP- 50+, PE- 17, MDN- 35

Replaces: 007- 17, SP- 2250, SPB- 50, SPB- 17, Rtx- 50 CPSil- 24, ZB- 50


BPX70 70% Dicyanopropyl 30% Dimethyl Polysilphenylene-siloxane

Cyano content of phase is equivalent to 70% cyanopropyl

Replaces: DB- 23, HP- 23, SP- 2330, SP- 2380, Rtx- 2330, 007- 23, PE- 23, CP- Sil 88, AT- Silar


BPX608 35% Diphenyl 65% Dimethyl Polysilphenylene-siloxane

Total phenyl content of phase is 35%

Equivalent to: DB- 608, SPB- 608, HP- 608, 007- 608, PE- 608


<< Feature of BPX Column

Application Areas >>

Application Areas

    Columns  Reason 
 

Mass Spectroscopy MS
(GC/ MS, GCQ, ITD, GC/ MSD, GCD)

  All BPX columns and HT columns for selected applications
  • Low column bleed characteristics
  • Extended temperature
  • Chromatographic inertness

Environmental
Semivolatiles, pesticides/ herbicides. aromatics/ PAH’s. drinking water volatiles and air monitoring

BPX5, BPX35, BPX50, BP624
  • Require low bleed columns, low detection levels
  • GC/ MS, ECD and NPD frequently used
  • Specific separations are required

Petroleum Industries, Oil Refineries

 

HT5, HT8, BPX1, BPX5, BPI PONA 
  • Analysis of high MW crude oils and residues
  • Gasoline samples require high resolution columns
Hospitals, Toxicology and Clinical Laboratories

 

BPX5, BPX35, BPX50, BPX70 (FAME anaylses)

  • Low bleed columns required
  • GC/ MS analyses frequently used
  • High operating temperatures offer an advantage in types of analyses performed
Food Products, Flavours/ Fragrances BPX70, BP1, BP20, BPX5, CYDEX- B   
  • Fatty acid methyl esters (BPX70)
  • BP20 for the analysis of free acids
  • Different polarities for finger printing
  • Enantiomeric separations

<< Capillary Column

BPX5 vs BPX50 >>

 

 

Confirmation Columns

  • Ideally columns will have different functionality in stationary phase
  • Have different relative retention between peaks of interest
  • Give adequate resolution of all solutes under the same experimental conditions
  • BEST RESULTS are shown when elution order changes between columns

BPX5 and BPX50 Phase Comparisons

  BPX5 BPX50

Description

  5% Phenyl polysilphenylene- siloxane

50% Phenyl Polysilphenylene- siloxane

Temperatures

0.25 - 1.0 µm
-40ºC to 360ºC/ 370ºC

>1.0 µm
-40ºC to 350ºC/ 360ºC

0.1-1 µm
80°C to 330/350°C

<< Application Areas

 

 

Organophosphorus Pesticides

Organophosphorus Pesticides

 

Compounds:
1. 4-Chloro-3-nitrobenzotrifluoride
2. 1-Bromo-2-nitrobenzene
3. Tributylphosphate
4. Terbufos
5. Dioxathion
6. Phosphamidon
7. Chlorfenvinphos
8. Ethion
9. Famphur
10. Carbophenothion
11. Triphenylphosphate
12. Phosmet
13. Leptophos
14. Azinphos-ethyl  

 

 

 
Columns: 30m X 0. 25mm X 0.25 µm
Initial Temp: 45º C (1 min)
1st Temp Ramp: 30º C/ min to 200º C (0.1 min)
2nd Temp Ramp: 7º C/ min
Final Temp: 315º C (hold 10 min)
Injector Temp: 280º C
Splitless Time: 1 min
Carrier: He, 1 ml. min
Instrument: HP 6890/ 5973

 

 

BPX5
PN: 054145
BPX50
PN: 054761

 

  

 

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HT - Very high temperature

Features to look for when Purchasing a Column

  • Low bleed
  • Maximum temperatures
  • Column inertness
  • Resistance to chemical degradation, air and water
  • Produces needed results

SGE Stationary Phase

  • BP - Conventional technology
  • BPX - High performance / high temperature
  • HT - Very high temperature Capillary Columns

HT Capillary Columns

  • HT5 (non- polar) 5% Phenylpolysiloxane (equiv.)
  • HT8 (slightly polar) 8% Phenylpolysiloxane (equiv.)

 

HT Column Features: Carborane modified polysiloxane backbone restricts phase degradation pathways

m-carborane

Where - CB10 H10C - represents the meta-carborane unit

 

 

HT Column Features:

  • Columns are monitored for column bleed
  • High temperature polyimide outer coating

HT5 - (non- polar)

  • 5% Phenylpolysiloxane (equiv.)
  • Ultra High Operating temperatures:
    Aluminium 460º C/ 480º C
    Polyimide 380º C/ 400º C

HT8 - (slightly polar)

  • 8% Phenylpolysiloxane (equiv.)
  • Ultra High Operating temperatures:
    Polyimide 360º C/ 370º C

Benefit No. 1

Ultra low levels of column bleed - columns are monitored for column bleed at elevated temperatures.
(HT5 (aluminium coated) 430º C)

Benefit No. 2

Carborane unit provides separation of aromatic isomers not achievable using conventional polysiloxane phases.
(HT8 - substituted aromatics)

 

 

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SolGel columns

What is SolGel?

The sol- gel process involves an alkoxide precursor followed by condensation to yield a polymeric silicate network. The phase is encapsulated in this silicate matrix.

Sol is the name given to the colloidal particles

Gel is the name given to material as the viscosity increases

SolGel Phases

SolGel-1ms™

  • 0 °C to 340/360 °C
  • Equivalent to 100% dimethylpolysiloxane

SolGel-WAX™

  • 30 °C to 260/280 °C
  • Equivalent to all Wax type columns

 

 

SolGel Capillary Columns


Robustness of SolGel-1ms

  • Carrier gas turned off at 320° C
  • Column left to bake for 1 hour
  • Carrier gas turned on and test mix analysed
  • Sample with a pH of 2 injected into the column

RESULT

No Change in Chromatography

Properties of SolGel-1ms™ as a phase

  • Inherently inert
  • Bonds very strongly to the surface of fused silica
  • Very resistant to acids
  • No cross-linking contaminants

SolGel Summary

  • Method used to prepare low- bleed inert columns
  • No cross-linking contaminants
  • Controllable process