FAQs - Syringes

Frequently Asked Questions


eVol®

What are the volume ranges and precision for each eVol syringe?
Syringe capacity Volume range Precision - Coarse Precision - Fine
5 μL 0.2 μL to 5 μL 0.1 μL 0.005 μL
50 μL 2 μL to 50 μL 1 μL  0.05 μL
100 μL 4 μL to 100 μL 2 μL 0.1 μL
500 μL 20 μL to 500 μL 10 μL 0.5 μL
1000 μL 40 μL to 1000 μL  20 μL 1 μL
How often should eVol syringes be calibrated?

If you change from dispensing one type of liquid (e.g. water) to another type of liquid (e.g. ethanol) then to get the best possible results, we would suggest re-calibrating to take into account factors such as differences in viscosity.

Apart from this, how often the calibration needs to be done will probably be determined by whatever quality system (in-house or external accreditation) the user is operating under. For example how often do they calibrate any other pipetting devices?

eVol is designed that the user can easily calibrate it themselves using an appropriately accurate balance (in this case we would recommend that the balance would need a minimum resolution of ± 0.0001 g).

However it may be that the users quality system requires that the calibration be done by an appropriately accredited 3rd party. Any company that that offers a pipette calibration service should also be able to calibrate an eVol.

You may also refer to the Support Tool - eVol calibration worksheet.

What is the optimum flow rate when using MEPS with eVol syringes?

With the MEPS BINs we recommend that the flow rate during aspiration be limited to 15 μL/sec to prevent cavitation. This will increase analyte/sorbent contact time and extraction efficiency.

What aspirate and dispense speed is ideal for my application?

The eVol is supplied pre-loaded with default aspirate and dispense speeds for each syringe available; these have been set by experimentation with water. The default speeds per syringe are shown in the list below:

Syringe capacity Aspirate speed Dispense speed
5 µL 10 10
50 µL 8 10
100 µL 6 10
500 µL 4 8
1 mL 3 7

Each different liquid type has different ideal speed settings, dependent on liquid viscosity and needle bore diameter. The best way to determine the correct speed setting is by experimentation.

If the speed is too high on aspiration the liquid will cavitate or the coupling between the syringe plunger and the eVol will disconnect. Cavitation occurs when the pressure is reduced below the vapor pressure of the liquid, essentially the liquid boils. The reduction in pressure is due to the restriction in the needle working against the viscosity of the liquid. The vapor bubbles over time disappear but some aeration of the liquid will always remain. For very accurate dispensing it is vital that cavitation of any sort be avoided. Should the coupling between the syringe plunger and the eVol disconnect you will notice the plunger will stop earlier than expected, this sample should be discarded, the speed should be reduced and then retried.

Setting the speed too low on aspiration has no negative effect on the dispense other than increasing the time between separate dispenses. It is better to run a little to slow than risk running too fast and influencing the results.

If the speed is set too high on dispense the restriction in the needle, in combination with the viscosity of the liquid, cause’s significant back pressure to build up. This back pressure may overcome the available driving force of the eVol and the motor may stall. The stalling of the eVol does not damage the drive. The eVol can be re-zeroed at anytime should this happen by simply going into the Toolbox/Re-Zero menu and pressing the RUN button.

Setting the speed too low on dispense has no negative effect on the dispense other than increasing the time between separate dispenses. It is better to run a little to slow than risk running to fast and stalling the eVol drive mechanism.

You may also refer to the Support Tool - eVol aspirate/dispense speed calculator.

I have tried resetting the battery on my eVol but it still will not start up and will not charge

Lithium ion batteries work best if they are kept charged. Unlike Nickel cadmium batteries, they do not experience memory issues if they are not fully discharged before recharging. Like all batteries Lithium ion batteries do have a limited life. The battery in your eVol has an expected life of approximately 12 months. After this 12 month period you may notice that your eVol does not hold charge as it used to and needs to be charged more frequently.

The battery will eventually get to the state where it can no longer hold charge and needs to be replaced. Replacement batteries are available from your local distributor or Trajan office, and the replacement procedure is quite simple and straight forward.

To replace the battery simply unscrew the stainless steel screw on the back of the handle and remove the rear housing. Unplug the battery connector and remove the battery from its seat. Locate the new battery in the seat and secure, then reconnect the battery. Replace the rear housing and screw; be sure to not overtighten the screw as it can crack the plastic housing. It is recommended that after replacing the battery you give the new battery a full charge for 3-4 hours.

I am consistently getting bubbles in my eVol syringes. I have tried slowing the aspiration speed which has helped a little, but hasn’t solved the problem.

Organic solvents commonly have a low vapour pressure. This can lead to bubbles being drawn from the solvent if it is aspirated too quickly - cavitation.

The following short procedure (<60 seconds) has been successfully used to reduce cavitation by preparing eVol syringes for use with solvents with a low vapour pressure such as ether diethyl, petroleum ether, dichloromethane, heptane, hexane, ethanol, isopropanol, cyclohexane, chloroform, etc.

  1. Aspirate 2 syringe volumes with water.
  2. Aspirate 2 syringe volumes with alcohol (ethanol).
  3. Aspirate 1 syringe volume with chosen solvent (eg Hexane).
  4. Aspirate with chosen solvent (eg Hexane) as required.
I am using chloroform and the plungers disconnect or stick in the syringe barrel.

Insufficient cleaning of the syringe may cause the plunger to swell. Manually aspirate the syringe with deionized water until the plunger movement feels smooth again.

Always ensure the eVol syringe is thoroughly cleaned after use.

The battery does not appear to be charging and the screen is blank, it seems as though the battery is dead

Lithium ion batteries work best if they are kept charged. Unlike Nickel cadmium batteries, they do not experience memory issues if they are not fully discharged before recharging. Like all batteries Lithium ion batteries do have a limited life. The battery in your eVol has an expected life of approximately 12 months. After this 12 month period you may notice that your eVol does not hold charge as it used to and needs to be charged more frequently.

The battery will eventually get to the state where it can no longer hold charge and needs to be replaced. Replacement batteries are available from your local distributor or Trajan office, and the replacement procedure is quite simple and straightforward.

To replace the battery simply unscrew the stainless steel screw on the back of the handle and remove the rear housing. Unplug the battery connector and remove the battery from its seat. Locate the new battery in the seat and secure, then reconnect the battery. Replace the rear housing and screw; be sure to not overtighten the screw as it can crack the plastic housing. It is recommended that after replacing the battery you give the new battery a full charge for 3-4 hours.

Does the touch-wheel on the eVol work when wearing gloves?

The operation of the eVol touch-wheel is not affected when operated while wearing gloves.

The sensitivity of the touch-wheel and whether it makes a sound when scrolling can be selected through the toolbox menu as follows:

Setting/Menu/Options
Spinner sensitivity/Toolbox : Preferences : Spinner/Low/Medium/High
Spinner sounds/Toolbox : Preferences : Sounds : Spinner /On/Off

Is there a chart that shows the flow rate for the selectable 1-10 speeds?

Please download the Support Tool - eVol aspirate/dispense speed calculator.

How long or how much use (work hours) we can get out of a fully charged battery?

A fully charged battery allows for 2-4 hours of continuous dispensing use or approximately 3,500 dispensing cycles.

eVol can be run even with a depleted battery. If the battery is depleted the unit can be operated from an AC power supply by using the power supply supplied with the eVol Kit. It will take approximately 30 seconds of charging to begin running the motor.

Can I use different needle configurations with my eVol syringes other than those supplied with the kit?

Other needles can be used with the eVol syringes.

For the 5 μL eVol syringe there are a range of specific needles, please refer to the Part Number List - Replacement Needles (PDPN-1001-G).

For the 50 μL and 100 μL eVol syringes any 25-500 μL standard replacement needles can be used.

For the 500 μL and 1000 μL (1 mL) syringes any 1-2.5 mL standard replacement needles can be used.

Can eVol be used to aspirate and dispense viscous liquids?

Yes, the eVol can be used to aspirate and dispense viscous liquids. However the following must be considered:

  • A needle with larger ID will help prevent restriction inside the needle.
  • Lower aspirate and dispense speeds should be used.

Default speeds and pause settings can be saved in Syringe Methods that can be assigned to specific syringes or selected at any time. Up to ten (10) syringe methods can be created and stored with the following settings:

  • Method ID
  • Syringe volume suitable for this method
  • Aspirate speed
  • Aspirate pause
  • Dispense speed
  • Prime volume
  • Prime Aspirate speed
  • Prime Aspirate pause
  • Prime Dispense speed
  • Prime Dispense pause
  • Prime cycles

Syringe Methods could be set up for different liquid viscosities and selected when required. For a high viscosity liquid the Syringe Method would be setup to use a slower aspirate and dispense speed and longer pauses.

Are the eVol syringes gas tight?

Yes, all the eVol syringes have PTFE tipped plungers so they are gas tight.

Can you supply a certificate of calibration with the eVol kit?

Yes the eVol kit can be calibrated but calibration must be requested at point of order by adding CAL to the kit part number eg 2910200CAL.

Can my eVol syringes be supplied with a certificate of calibration?

Yes the eVol syringes can be calibrated. Calibration must be requested at point of order by adding 'CAL' to the end of the part number eg 2910020CAL.


Jumbo Syringes

Jumbo syringe o-ring dimensions

Dimensions:

  • 500 mL 0.22 x 2.283" OD (part number 032527)
  • 1000 mL 0.22 x 3.52" OD (part number 032532)
  • 2000 mL 0.22 x 4.646" OD (part number 032537) 
What are the O-rings made of?

The O-ring material is FEP (fluorinated ethylene propylene).

Is benzene compatible with the Jumbo syringe?

Our Jumbo syringes are not suitable for use with benzene.

Can Jumbo syringes be used with hydrocarbon gases?

We would not recommend the Jumbo syringe for this application as the acrylic barrel could become brittle leading to cracks forming.

What is the maximum pressure for a Jumbo syringe?

The acrylic barrel will handle 30 psi. However to achieve this would require a force on the plunger of over 500 pounds.

What is the maximum temperature for a Jumbo syringe?

The maximum temperature for the Jumbo syringe is 80°C.

My Jumbo syringe is leaking, what should I do?

There is a nut at the rear of the plunger disk that, when tightened, presses the O-ring outward. To reach this nut remove the back cover of the syringe and withdraw the plunger. About a quarter of a turn of the nut should be enough to give a better seal. Care should be taken re-inserting the plunger so as not to damage the O-ring.

Is it possible to treat the inner surface to make the Jumbo syringe barrel inert?

We are unable to coat (or treat) the inside of the acrylic barrel.

The plunger action on my Jumbo syringe does not feel smooth

Smooth plunger action is achieved by smearing a small amount of silicone oil (350 centistokes viscosity) on the O-ring. Ensure any excess is removed once the plunger action feels comfortable.


Syringes

What are the removable luer lock fittings made of?

On the removable luer lock syringes the luer cone is Kel-F and the metal part is nickel plated brass.

How do I connect tubing to the needle end of my syringe?

If the tubing is flexible and has an ID similar to the OD of the needle (you may be able to change needles to achieve this), then the simplest thing to try is pushing the tubing over the needle. Be very careful if it is a bevel tip needle as it may slice through the wall of the tubing. A cone tip or dome tip needle will make it easier.

If the tubing doesn't have the right ID, or if it isn't flexible enough, luer lock syringe (or one with a 1/4-28 UNF male thread) can be used along with a luer lock (or screw thread) adaptor.

When should I use a syringe with a valve on it, rather than a standard gas tight syringe?

If the gas being sampled is under pressure then you definitely would want to use a syringe with a valve, otherwise some of the sample will escape.

It is also a good idea to use a syringe with a valve if the sample, once taken, is not going to be immediately introduced into the GC.

Other possible reasons for using a valve are, very low level samples, very concentrated samples, toxic, corrosive or malodorous samples, or if you need to pressurize sample prior to injection.

What is the cleaning solution in the Needle Cleaning Kit (part number 031782)?

The cleaning solution is Extran. The solution is biodegradeable and is phosphate free.

What is the maximum temperature for manual syringes?

Fixed needle, fixed luer lock, and 500 nL - 5 μL NanoVolume syringes can be heated in an oven to 70°C

Removable and fixed needle 5 - 500 μL all metal plunger syringes can be heated in an oven to 120°C

Removable needle and removable luer 1 mL - 100 mL PTFE Tipped Plunger and Dispenser syringes can be heated in an oven to 120°C

Note: Plungers should be removed before heating. Ensure heating and cooling is a gradual process to prevent the glass barrel from breaking

Would you recommend putting a syringe in the autoclave?

Syringes can be autoclaved but we would recommend the following procedure:

Autoclaving will usually heat a syringe to about 121°C so that is too high and usually the cool down is speeded up by purging the system too early and that results in the syringe breaking. However it is generally possible to reduce the autoclave temperature to 100°C, extend the time, and there is usually a setting to allow for a slower cool down.

Note: Plungers should be removed before autoclaving. 

What grade of stainless steel are the needles made of?

Our standard needles are grade 304 stainless steel.

Are all solvents compatible with my fixed and removable needle syringes?

Some solvents will gradually dissolve the glue used to hold the back flange and the front flange (fixed needle or removable needle thread). It's a matter of limiting the syringe's exposure to the solvent (both the length of time and the area exposed).

Do not immerse front flange below the level of the solvent. After use rinse syringe with clean solvent, followed by acetone. Then disassemble the syringe and allow it to dry. 

What is the maximum pressure for the syringe valves?

The maximum pressure is 100 psi.

What is the maximum temperature limit for B type (plunger in needle syringes)?

The maximum temperature limit for B type (plunger in needle syringes) is 70°C.

What are the fitting sizes on removable needle syringes?
  • 5-10 μL syringes - 10-32 UNF (not 5 μL NanoVolume)
  • 25 μL to 2.5 mL - 1/4-28 UNF
  • 5 mL to 2 L - 7/16-28 UNEF 
How do I select a replacement needle?

Needle length refers to the exposed length of the needle when fitted to syringe. Choose a replacement needle by selecting the syringe volume, needle length, OD and tip style.

For example, a 10 µL MicroVolume syringe part number 002050) is supplied with a 50 mm bevel tip needle (part number 037110). This can be replaced with the same needle, or any needle designed for 10 µL syringes.

What are the syringe scale graduations for 1 - 100 mL syringes?

Syringe capacity (mL)

Major scale (mL)

Minor scale (mL)

1

0.1

0.02

2.5

0.5

0.05

5

0.5

0.10

10

1.0

0.20

25

1.0

0.50

50

10.0

1.00

100

10.0

2.00

What is the part number for the compression spring in my removable needle syringe?

Part number 032030 for a pack of 10.

What is the Luer cone in luer lock syringes and luer lock syringe valves made of?

The luer cone is made of Kel-F.

Can I purchase the luer lock fitting as a separate item?

Yes, the replacement luer lock fitting (Kel-F luer cone and threaded metal adaptor) is part number 031902. The replacement Kel-F luer cone is part number 031903

The plunger on my new syringe is different to the plunger on the syringe I have been using in my autosampler

The plungers for 5, 10 and 25 μL syringes for CTC (not RTC) and Thermo TriPlus (not RSH) syringes were redesigned to prevent damage to the syringe barrel under certain conditions. Replacement plungers can be adjusted by locking the button in position against the back flange (using the small hex screw) when the plunger is in the zero position.

I have purchased a new syringe for my autosampler but it does not fit as the plunger button is too big.

The 5, 10 and 25 μL syringes for CTC (not RTC) and Thermo TriPlus (not RSH) will not fit in the following autosampler models: Thermo (AS3000, AS2000, AS200/800), CTC A200S, Varian 8400 and Antek 9000 A200SE. For the correct syringes please refer to (link for Thermo GC syringes)

Can my syringes be supplied with a certificate of calibration?

Yes all our syringes can be calibrated. Calibration must be requested at point of order by adding 'CAL' to the end of the part number eg 002000CAL.

Do you offer a syringe calibration service?

We can only offer a calibration service at point of order. Once the syringe has left the factory we are unable to calibrate the syringe.

Are the V6 dispenser syringes such as 003696 suitable for use in Eksigent and Dionex Nano HPLC autosamplers?

No, although they look very similar, they slightly too long to fit.

Why are the needles on our CTC headspace syringes a different length to those on the ones from Hamilton?

This difference comes from the difference in the design of the syringe where the needle is fitted to the barrel, as shown in the image below. It seems that Hamilton’s 65 mm is just the length of the needle itself and does not include the extra length of the glass Luer tip on the end of their syringe barrel.

I am using a syringe pump and need to calculate the pumping speed. What is the ID of my syringe barrel?

Syringe capacity

Needle type

Syringe barrel ID (mm)

Example part number

5 μL

Removable

0.343

001310

10 μL

Removable

0.485

002310

10 μL

Removable

0.485

002312

25 μL

Removable

0.728

003312

50 μL

Removable

1.03

004312

100 μL

Removable

1.457

005312

250 μL

Removable

2.303

006312

500 μL

Removable

3.257

007312

1 mL

Removable

4.606

008105

1 mL

Removable

4.606

008025

2.5 mL

Removable

7.284

008505

2.5 mL

Removable

7.284

008425

5 mL

Fixed

10.301

008762

10 mL

Fixed

14.567

008962

25 mL

Fixed

23.0

009463

50 mL

Fixed

27.5

009660

100 mL

Fixed

35.0

009760

Can I change the needle on a calibrated syringe and maintain the calibration?

With the exception of the 0.5 μL, 1 μL and 5 μL NanoVolume syringes, SGE syringes are calibrated using the internal dimension of the barrel, rather than measuring the volume that the syringe delivers. SGE syringes are therefore calibrated to contain, not calibrated to deliver. Changing the needle won't change the volume contained in the syringe barrel, however it may change the amount delivered. To determine any effect of a needle change on the delivered volume, you should measure the delivered volume gravimetrically, or with a spectrophotometer.

Can I check the dispensed volume of a syringe using a photometer?

The photometric method can be used as an alternative to gravimetric calibration of volume measuring devices (above). A solution (usually a dye) of known absorbance at a particular wavelength is diluted to a known volume using the syringe to be calibrated. The theoretical dilution value (ratio of syringe volume to final volume) can then be checked against the actual dilution value (ratio of absorbance of initial solution to absorbance of diluted solution). This method is limited by the absorbance range and accuracy of the photometer and the accuracy to which the volume of diluent can be measured. This method is limited by the absorbance range and accuracy of the photometer and the accuracy to which the volume of diluent can be measured. Care must be taken with dye solutions, as they may stain the glass barrel of the syringe.

What cleaning methods and maintenance procedures should be followed to ensure the calibration certificate remains valid? Do you recommend a special cleaning method, or a temperature at which these syringes should be kept? What should be avoided?

Calibrated syringes do not require different storage, cleaning, or handling to normal syringes. Anything that affects the accuracy and reproducibility of a normal syringe will also affect a calibrated one. The only particular thing to avoid would be altering the setting of a repeating adaptor, where the calibrated syringe has one. A calibration certificate simply states that a syringe was made with certain internal dimensions and was delivered to the customer with those specifications. Good laboratory practice dictates that wherever possible syringes should be used at a constant (room) temperature for maximum accuracy and reproducibility.

What is the easiest way to check the dispensed volume of a syringe?

This is done by accurately weighing the amount of pure water that the syringe delivers (and recording the results a number of times). You will need an analytical, or semi-micro, balance in a temperature controlled environment, free from vibration. Draw up the water and dispense it exactly the same way a number of times. Wipe the outside of the needle so that no water adheres to it, taking care not to draw any water out of the needle itself.


MEPS

Are the MEPS BINs reuseable?

The MEPS BINs can be reused 40-100 times but this will depend on the matrix.

What weight of phase material is in each MEPS BIN?

Each MEPS BIN contains 4 mg of phase.