Training - GC basic problem solving

• Do not heat until purged with oxygen-free gas (10 volumes)
• Always note injector and detector ends
• Always cut end of column after passing through ferrule
• Consider column dimensions and phase
• Consider the use of pre-column
• Set correct flow rate using linear carrier gas velocity
• Do not overheat column unnecessarily
• Use good quality ferrules and change as needed
• Support the column cage in the oven

• Very large volume injections
• Dirty samples
• Very acidic/basic samples
• Poor gas management
• Overheating

• Remove 50 cm to 1 m from the injector end of the column
• Turn the column around and re-condition it
• Wash the column

Compounds:
1. Decane
2. 4-Chlorophenol
3. Decylamine
4. Undecanol
5. Biphenyl
6. Pentadecane

Choose a conditioning temperature 20°C above your method temperature, or choose the Maximum Specified Temperature - whichever is the lower.

Remember: the lower the temperature, the longer your column will last.

• Store the column so it can’t be scratched
• Seal the column ends (with septa)