Download: Application Note - Immobilized pepsin digestion of Cytochrome-C [PDF]
水素重水素置換反応装置
フレキシブルな機能
Contact a workflow specialist for your specific needs
Since 2002 Trajan has been automating HDX, providing continuous enhancements for rugged HDX sample control and scheduling.
Trajan’s LEAP HDX system is the recognized leader, preferred around the globe by scientists requiring confidence in reproducible results.
Automated HDX provides structural confidence in native states
HDX, especially automated HDX, has uses in development, manufacturing and post-production, providing insight into your protein’s tertiary structure, in its native state(s).
Successful HDX experiments are all about control
With robotic precision, Trajan’s HDX platform exerts exacting control of all key factors:
HDX is a well-established analytical technique for protein-ligand, protein-protein, protein-conjugate interactions and protein stability studies.
HDX uses variations in deuterium exchange rates between more and less accessible/structured regions throughout a protein.
In addition to being faster than other protein techniques like x-ray crystallography and NMR, HDX data can be obtained from samples that are challenging to crystalize and from samples in short supply. It can be used to replicate critical native state conditions, for the most useful results. HDX is an indispensable complementary tool for protein biophysics.
Trajan’s HDX system is compatible with bottom-up, top- and middle-down protein analysis studies.
The total HDX platform
Our total HDX platform covers the complete process: sample input >> the many meticulous steps of preparation >> optimizing mass spec utilization >> clear answers with minimal manual data curation.
Faster answers
Powerful information on protein structure in days – not weeks. Move more quickly, make better decisions.
With HDExaminer, swiftly and easily analyze data from any MS instrument to get a complete picture of the overall deuteration behavior of your protein.
Automation at its finest
The most efficient system control, with unique temperature set points at all stages of the experiment to ensure reliable data. Sustained 0°C environment reduces back exchange, improves sample stability and overall reproducibility.
Our Chronos for HDX software offers a streamlined interface for HDX users and automatically overlaps time points for maximum MS efficiency.
Combines ease of use with advanced options, allowing promotion of user-defined parameters to the sample list, giving the operator control over all aspects of the method without complication.
HDExaminer: a complete analytical tool for HDX experiments
The Trajan LEAP HDX platform works natively with HDExaminer from Sierra Analytics, a complete software solution for HDX analysis, independent of MS data type.
HDExaminer is designed with ease of use in mind, offering numerous ways to visualize your data. These include:
Unique fluidics automation for the best performance
Trajan’s LEAP HDX platform incorporates an advanced cooling chamber for the trapping and chromatography phases which includes an enclosed valve and column chiller as well as solvent pre-conditioning.
Digestion temperature is controlled independently with the protease column chamber, to allow improved digestion efficiency.
A special 3 valve configuration enables zero-downtime backflushing for retention time stability, extended column life and superior carryover protection.
Optimized fittings and precut tubing ensure maximum reliability and instrument up-time.
Specifications |
Compact |
Base |
Parallel (standard) |
Parallel (extended) |
---|---|---|---|---|
Automatic syringe selection for extended dynamic range |
✔ |
✔ |
✔ |
✔ |
Chilled syringe for quench/injection |
✔ |
✔ |
✔ |
✔ |
Vial capacity for 100 time point experiments |
✔ |
✔ |
✔ |
✔ |
Optimized valve fluidics for inline digestion and automatic backflushing |
✔ |
✔ |
✔ |
✔ |
Exchange time points down to 30 seconds |
✔ |
✔ |
✔ |
✔ |
Independent protease column temperature control |
✔ |
✔ |
✔ |
✔ |
Trajan HDX syringes |
✔ |
✔ |
✔ |
✔ |
Trajan ProDx protease columns optimized for low temperature |
✔ |
✔ |
✔ |
✔ |
Trajan ProDx trap columns optimized for low temperature |
✔ |
✔ |
✔ |
✔ |
Dual head for simultaneous operations |
- |
- |
✔ |
✔ |
Exchange time points down to 15 seconds |
- |
- |
✔ |
✔ |
Forward compatible capacity for future models |
- |
- |
Optional |
✔ |
Secondary chilled syringe for protein handling |
- |
- |
Optional |
Optional |
Membrane protein filtration |
- |
Optional |
Optional |
Optional |
96 or 384 well microtiter plate capacity |
Optional
|
Optional |
Optional |
Optional |
Expert training
Trajan Scientific and Medical is the global expert in HDX automation. Our in-house specialists and experienced partners offer the best training and consulting available to achieve your HDX goals.
Supporting more operation modes in more places than ever
With 40 years combined experience in HDX and coupled to our unparalleled relationships with the global leaders in the technique worldwide, our HDX automation continues to offer application support for an ever-expanding portfolio of options.
New features and integrations now offer you better access to membrane proteins, proteins at biologically relevant temperatures, and even more flexible protein regions than ever before.
The Trajan LEAP HDX platform is supported directly by Trajan in Europe, Australia, Japan and the USA, and through partners in the Middle East, India and beyond.
Service you can trust
Our CTC manufacturer-certified service team is available to assist you in person, by phone and remote session.
Now offering local time zone support in US, EU, ANZ and Asiatic time zones.
Available extended support contracts, training and preventative maintenance schedules.
Consumables designed for HDX
Columns and fluidics specifically designed to optimize all aspects of the HDX user experience. From ease of use to reliability and sample stability, your success is our number one goal.
ProDx Protease columns are designed to perform with lower carryover, lower backpressure, and better digestion characteristics at 0°C for longer lifetimes.
ProDx Trap columns are uniquely engineered to operate under HDX experimental conditions.
View the range of ProDx columns
Download: Performance Report - ProDx Protease column [PDF]
View full product details
Download: Product Data Sheet - ProDx Protease columns [PDF]
Also see: Part Number List - ProDx columns
View full product details
ProDx consumables are specifically designed and developed for use with the LEAP HDX platform.
The ProDx Protease column is optimized to perform at 0°C, the ideal temperature for performing HDX reactions.
Improved protease coupling reduces carryover from non-specific binding, while lower backpressures minimize the potential for contamination and overpressure leakage.
When used in combination with the new LEAP HDX valve system, it can be backflushed automatically to remove frit-loaded or undigested intact material. Clean columns give consistent retention times, overcoming traditional issues with non-selective binding and poor reproducibility.
Automated Hydrogen-Deuterium Exchange (HDX) experiments using the LEAP HDX platform.
ProDx columns were developed specifically for use with the LEAP HDX system. Packed in bio-inert stainless steel glass lined columns to withstand high pressure digestion practices, making it compatible with other systems.
The ProDx Protease (Pepsin) column is the first in a range of immobilized Protease columns available from Trajan. The initial intended use is with the LEAP HDX workflow, where these columns are used in-line for protein-protein and protein-ligand studies. The columns can be deployed outside of the HDX workflow as appropriate, possible alternate configurations might include general proteomics workflows, protein purification studies and QC applications. Column chemistry is such that limited exposure to common denaturing reagents and quenching salts (such as 6.4M Guanidine Hydrochloride, 8M Urea and 4M TCEP) do not adversely impact proteolytic performance.
ProDx Protease (Pepsin) columns are delivered chilled for optimal performance. Columns need to be maintained between 2-8°C. Column performance can be reduced if the column is maintained and operated above 15°C for extended periods of time, or if exposed to non-acidified aqueous reagents or organic reagents.
Phase | Pore size (Å) | Particle size (µm) |
Protease (Pepsin)* | 500-10,000 Å | 20 μm |
Please refer to the part number list for more items.
*This product contains POROS® material used under license for Sample Prep purposes from Life Technologies Corporation, a subsidiary of Thermo Fisher Scientific, Inc. The POROS® Trademark is owned by Thermo Fisher Scientific, Inc.
View full product details
Download: Technical Poster - Automated Hydrogen-Deuterium Exchange with optimized consumables [PDF]
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