HDX has become an indispensable tool for protein binding studies in pharmaceutical development. For over 15 years Trajan's automation team (formally LEAP Technologies) have been automating HDX experiments, and is the industry leader in the field.
LEAP HDX systems are helping researchers across the globe come to answers about their proteins faster than traditional methods.
Powerful information on protein structure in days - not weeks. Move more quickly, make better decisions.
Automated Hydrogen-Deuterium Exchange (HDX) experiments.
Our turn-key HDX solution is compatible with most MS analyzers, and can extend your current LC/MS capabilities while adding a new dimension to your protein studies.
LEAP HDX automation
The LEAP HDX platform is an advanced, automated scheduling and experimental workstation providing ease of use, high reproducibility and exceptional data quality for HDX experiments.
HDX relies on accurate timing of the labeling step.
The system will optimize the timing of the labeling step to ensure quenching is just in time for the LC-MS injection, using a chilled syringe. A typical experiment might consist of a dozen time points, in triplicate, for each protein sample. The LEAP HDX system will schedule the experiments and perform all steps for each time point of every sample. Long labeling experiments are automatically scheduled first to ensure the shortest possible instrument time.
Optimized HDX fluidics
The introduction of optimized HDX fluidics that enables full fluidic path backflushing, extending column life and maintaining consistent retention times to deliver higher quality data.
The new fluidics pathway is built upon UHPLC rated valves and column hardware (21 kpsi), with flow paths minimized to reduce swept volumes.
Trajan manufactured HDX specific pre-cut, high pressure lines and fittings, designed to improve reliability and enhance performance. All fluidic components are designed to offer consistently reproducible results.
ChronosHDX control software
Meeting the challenge of producing the highest quality data sets.
HDX experiments require time-sensitive operation. Therefore, in traditional methods, an operator has to attend throughout the course of HDX experiments. However, time-controlled automation enables unattended operation using intelligent scheduling with Chronos software.
Trajan uses ChronosHDX scheduling software to automate the precise labeling and quenching of samples, as well as injection and digestion of proteins on the LC-MS analysis system.
Native data format compatibility with HDExaminer increases high confidence data and allows you to get to results faster.
Efficient system control and optimized experimental design
Our Chronos for HDX software now offers a streamlined interface for HDX users and automatically overlaps time points for maximum MS efficiency.
Temperature control at all steps ensures reliable data. Sustained 0°C environment reduces back exchange, improves sample stability and overall reproducibility.
Our most flexible automation platform yet offers the ability to run multiple experimental methods in the same sequence. Native data format compatibility with HDExaminer increases high confidence data and allows you to get to results faster.
|LEAP HDX specifications|
|On-exchange time range||10 seconds to 4+ hours, user selectable|
|Temperature range||0°C to 37°C|
|Volumetric range||Typically 1-250 μL, use application selectable to 5000 μL|
|Electrical||120 VAC, 60 Hz or 220 VAC, 50 Hz|
|Dimensions||80 cm (W) x 38.5 cm (D) x 64.8 cm (H)|
|LCMS data systems||Compatible with nearly all LCMS data systems. Direct integration with the most common.|
Service you can trust
Our CTC Manufacturer Certified Service team is ready to keep your automation at its peak performance.
Full application support
Trajan is the leading expert in automation of HDX. Our in-house specialists and team of experienced partners offer you the best consulting available to achieve your HDX goals.
Trajan has the expertise and experience with automating HDX experiments that can only come from having worked with leading scientists in the field.
ProDx consumables designed for HDX
Columns and fluidics consumables specifically designed to optimize all aspects of the HDX user experience.
View the range of ProDx columns
High-throughput, high sensitivity and low carryover: the new infusion automation from Trajan offers you everything you need to bring your lipidomics mass spec workflow to its peak performance.
Move from manual sample infusion to the highest standard of infusion automation. With rapid cleanup between injections and best-in-class software architecture to integrate into your workflow, our infusion automation will elevate your lipidomics experiments.
The most consistent and flexible system available for infusion automation
Fully customizable infusion eliminates carryover concerns while saving you time. Allows for sample infusion at flow rates between 5 and 100 µL/min — directly into your ESI source via the injection valve — precisely and without pulsation. Compatible with both shotgun and targeted lipid analysis.
The powerful Chronos software can execute important operational and integration functions such as reading and reporting to LIMS software, as well as MS acquisition mode switching within a single infusion. The system maintains flow to the source while quickly and thoroughly washing the syringe system for the next infusion.
Exclusive inert configuration is available for your trickiest lipid classes — ensuring your samples never come into contact with any polymers.
“We just ran a whole pile of samples. It ran 24/7 without a hitch. Very reliable.” - Calvin Vary, PhD, Proteomics and Lipidomics Core Facility Maine Medical Center Research Institute, Maine, USA.
Upgrade sample throughput without worrying about carryover
The LEAP PAL injector head is a syringe pump itself, making it the ideal sample delivery system for ESI flow rates, unlike conventional HPLC pumps.
Many lipids are notorious for their adherence to glass and other surfaces resulting in carryover between samples. The special LCMS syringe tool incorporates unique features to minimize this carryover to below 1% for the abundant neutral and phospholipids and even lower for other species.
The illustration below shows how this rapid washing is achieved. After washing the inside, the system will wash the needle externally and be ready to aspirate the next sample.
Figure 1 Sample infusion
Greatest experiment control with powerful, user-friendly software
The infusion process is controlled using our Chronos software, allowing maximum compatibility with your preferred Chromatography Data System, LIMS and MS data systems.
Method parameters are available for flow rates, infusion times, stabilization delays and cleanup conditions, MS acquisition method switching.
With an optional second valve, standard chromatography methods can be used in the same sequence with direct infusion samples.
Figure 2 An example Chronos infusion method
|Flow rate range||5-100 μL/min.|
|Sample formats||Sealed and unsealed vials (1 mL, 2 mL, 10 mL); sealed and unsealed plates (96 and 384-well).|
|Optional features||Sample cooling drawers (4-40°C), inert flow path, second valve for chromatography autosampling|
|Sample capacity||Expandable capacity, from three plate tray holder, to 6 or 12 plate drawer stack(s). Capacity depends on vial/tray formats and drawer configuration, maximum 4 stacks|
|Signal stability||Signal stability on a single sample has been demonstrated for 20 minutes. Long term stability confirmed in successful analysis of 400 samples over a 36 hour period (5 minute injection per sample).|
|Computer requirements (minimum)||Operating system: Windows 7 SP1 (32 or 64 bit), Windows 8.1 or Windows 10. Hardware: Intel Dual Core 2.0 GHz, 60 GB hard disk, 4 GB RAM, dedicated ethernet port or free location on local private network switch with 1 free USB port for security dongle. Monitor: 1366 x 768 (recommended 1440 x 900 or 1920 x 1080).|
Zero-loss, zero-drip, high throughput and ultra reliable: the new microfraction collection system from the Automation team at Trajan (formally LEAP Technologies) offers you everything you need to consistently perform your most critical collection experiments.
The LEAP MFx collector has been purposefully designed to optimize fraction collection for the sharpest peaks, the maximum dependability, and the greatest ease of use. With smart software features and automation, this analytical fraction collection system is the most reproducible, and highly compatible platform available today.
The highest capacity, most flexible system available
Fully customizable collection into a variety of plate and vial types, including sealed container collection: 96 and 384 well plate formats, 2 mL and 10 mL vial formats (sealed or open), and 2 mL and 10 mL tube formats.
Compatibility with a broad range of analytical flow rates, sub-ambient temperature control, and user-friendly software are just a few of the features that make this new system the perfect evolution for your fraction collecting needs.
Automated microfraction collection experiments.
The most flexible system on the market, the LEAP MFx collector solution is available:
|Plate capacity||Up to 12 plates||Up to 24 plates|
|Instrument width||50 cm||120 cm|
Designed for safety and serviceability
|LEAP MFx collector||Competitor 1||Competitor 2|
|Zero-loss collection (doesn’t divert to waste)||Yes||No||No|
|Cooling to 4°C||Yes||Yes||No|
|Deep well plates||24||4||8|
|Shallow well plates||48||4||8|
|Vials/tubes||24 trays (up to 1296 tubes/vials)||215 tubes||768 tubes|
Unique dynamic flow reservoir
The precision engineered and long lasting dynamic flow reservoir (DFR) allows the LEAP MFx collector to continue collecting chromatographic flow while the automation is moving between wells, resulting in zero-loss.The design achieves a turbulence-free, fully optimized flowpath for collection without peak dispersion.
Undisturbed flowpath for minimal peak diffusion
While flow to the buffer region remains constant during both well filling and robot movement, the buffer region will expand as the DFR travels between wells. This retracts any hanging droplets, and avoids peak diffusion by allowing continuous flow. The buffer region contracts to resume dispensing at the next well.
Setup for collection has never been easier
Tabular and visual method creation
Maintains highest peak resolution with sub-2 second fractions, while providing simplified data correlation, and advanced sample tracking
Smart features offer convenience and reduce error
|LEAP MFx collector specifications|
|Maximum flow rate||>5 mL/min|
|Minimum dwell time||<2 seconds/well|
|Collection formats||96 and 384 well plates (deep and shallow), 2 mL and 10 mL vials/tubes|
|Sealed collection||Can pierce sealed plates and sealed vials|
|Computer requirements (minimum)||Operating system: Windows 7 SP1 (32 or 64 bit), Windows 8.1 or Windows 10.
Hardware: Intel Dual Core 2.0 GHz, 60 GB hard disk, 4 GB RAM, dedicated ethernet port or free location on local private network switch with 1 free USB port for security dongle.
Monitor: 1366x768 (recommended 1440x900 or 1920x1080).
Service you can trust
Our CTC Manufacturer Certified Service team is ready to keep your fraction collection automation at its peak performance.
Full application support
Trajan is the leading expert in automation of fraction collection for analytical pursuits. Our in-house specialists and team of experienced partners offer you the best consulting available to achieve your microfraction collection goals.
Trajan has the expertise and experience with automating fraction collection experiments that can only come from having worked with leading scientists in the field.
Reliable engineering and consumables for sharp peaks and reproducibility
Tubing replacement kits with pre-cut tubing, optimized fittings and ferrules ensure that the MFx collector, built on the trusted PAL platform, is the most advanced fraction collection automation for zero carryover, sharper peaks and stunning reproducibility.
View the range of LEAP MFx collector accessories
An example of actual sample collection with the LEAP MFx collector, showing baseline resolution and well-defined peaks
The newly updated LEAP NMR prepstation is a modular solution designed to help you improve your sample preparation workflow.
From basic workflows...
To complex sample handling...
View full product details
Powered by Chronos, the NMR prepstation can be configured as a walk-up, push button system, or allow the user to build custom sequences in the full software interface.
The PAL3 System is one of the most widely used and successful sample preparation and handling platforms.
PAL System’s new generation of sample preparation and handling instruments.
The LEAP CHRONECT® Quantos integrated powder-liquid automation system is a complete workflow like no other. This powerful platform is achieved through seamless coordination of industry proven, trusted, modular elements.
The high-precision, safe and reliable powder automation you’ve always wanted.
Integration of accurate dosing and weighing technology into liquid sample preparation maximizes your time and resources while minimizing user exposure and error sources.
The most complete, trusted and flexible system available
The industry-leading Quantos powder dosing capability, from METTLER TOLEDO, enables the highest level of accuracy and safety in powder delivery. The calibrated and certified balance is isolated from the moving components through optimized mass and mechanical damping. The integrated, multi-axis, cooperative robot combined with the precision-engineered platform facilitates the automation of up to 32 different powders.
Synchronized and in parallel, the PAL workstation automatically seals and caps vials, adds accurate liquid volumes, agitates, vortexes, mixes, heats, and or promotes dissolution or extraction from the solid, according to your workflow.
Positive tracking and traceability of powder dosing heads through RFID
Positive tracking and traceability of powder dosing heads is achieved through embedded RFID tracking. This tracking and workflow information can be output directly to LIMS systems, data systems, PDF, Excel or CSV formats.
The powerful Chronos software empowers users with familiar sample/method based control. The Chronos software natively drives all modules in the system, eliminating any need for robot programming in your laboratory. Chronos also expands compatibility to integration with large scale automation systems, all major analytical instrument data systems, and legacy QX96 excel files.
Compact footprint allows you to handle powder samples in a safe, inert gas environment
The integrated system is designed with your lab in mind, and a footprint compatible with benchtop, open laboratory environments and both positive and negative pressure cabinets.
Powder can be added to vials in a completely isolated environment, sealed in inert gas, and prepared for any analytical technique, without users coming into contact with potentially toxic or otherwise hazardous powders.
Sample and dosing
Powder dispensing range: 1 mg - several grams.
Component dosing heads: Up to 32 Quantos standard dosing heads.
Suitable powders: Free-flowing, fluffy, granular or electrostatically charged.
Dispensing time - 1 component: 10 - 60 seconds, depending on compound.
Target vial formats: Sealed and unsealed vials (2 mL, 10 mL, 20 mL); unsealed 1 mL vials.
Dimensions (w x d x h):
- without capping or liquid prep: 950 mm x 650 mm x 825 mm
- with capping and/or liquid prep: 1575 mm x 795 mm x 825 mm
CHRONECT robot: cooperative robot and Quantos platform.
Quantos powder dosing: automated analytical balance and dosing module.
Capping/liquid automation: PAL system and Chronos control software - varies by application.